作者：Li P 翻译：孙琳 校对：姚中强

摘要：目的：复发性流产（RSA）的发生率日益增加，严重影响女性的生殖健康。免疫系统在RSA发生中的作用受到研究者的关注。Toll样受体4（TLR4）和趋化因子CCL2与多种妊娠疾病相关。本研究的目的是明确TLR4和CCL2水平与RSA的相关性，并探讨其能否作为RSA的标志物。

方法：采用病例对照研究，纳入36例RSA患者和36例健康对照，收集蜕膜及绒毛组织和静脉血。荧光定量聚合酶链反应用于检测TLR4和CCL2 mRNA表达情况，酶联免疫吸附法检测其血清水平表达。

结果：与健康对照相比，RSA患者TLR4和CCL2组织mRNA水平及血清蛋白水平表达均显著增加（p<0 .05）。相关性分析结果显示，血清及组织mrna水平tlr4呈正相关，ccl2同样存在血清水平及组织mrna水平正相关。

结果：TLR4和CCL2与RSA的发生密切相关，提示TLR4和CCL2可能成为孕妇发生RSA的监测指标。

附全文：Abstract The increasing incidence of recurrent spontaneous abortion (RSA) severely affects women's health. The involvement of the immune system during pregnancy and its contribution to RSA draw researchers' attention. Both Toll-like receptor 4 (TLR4) and chemokine (C-C motif) ligand 2 (CCL2) have been linked to various pregnancy disorders. In this study, we quantified TLR4 and CCL2 levels in a case-control study in order to elucidate the correlation between these factors and RSA, and the potential to use them as disease markers. A total of 36 RSA patients and 36 healthy control individuals were recruited for the donation of decidual and chorionic tissues and venous blood samples. Fluorescent quantitative-polymerase chain reaction was used to measure mRNA levels and enzyme linked immunosorbent assay was used to quantify serum levels of TLR4 and CCL2. RSA patients had higher TLR4 and CCL2 mRNA levels compared to controls (P < 0.05). serum levels of the two factors were also significantly higher in rsa patients than in the control group (p < 0.05). positive correlations were found between serum levels and tissue mrna levels of tlr4 and ccl2. in conclusion, both tlr4 and ccl2 were closely related to the occurrence of rsa, suggesting that serum tlr4 and ccl2 levels could be used as indices for monitoring rsa in pregnant women.

引自：Li P, Wu HL, Dong BH. Relationship between TLR4 and CCL2 expression and recurrent spontaneous abortion. Genet Mol Res. 2016 Jan 8;15(1).